Research Publications - 2011 - Agriculture and Agri-Food Canada (AAFC)
The publications in this list are: articles presenting original research, published by third-parties (non AAFC) and appear in peer-reviewed scientific periodicals or in peer-reviewed books
The plasma membrane is asymmetrical; glycolipids and proteins occur only on outside and cytoskeletal filaments attach to peripheral proteins only on the inside surface. Discuss the fact that South America was inhabited by marsupials until relatively recently (within the last 20,000 years or so), when the land bridge formed between North and South America, which allowed placental mammals to reach South America and led to the extinction of many marsupials
abdomen study comparing: Topics by Science.gov
We present a case of acute abdominal pain due to torsion of wandering spleen in a patient with Marfan Syndrome, valvular heart disease, and vertebral anomalies. This paper presents a comparative experimental study of various reconstitution techniques and evaluates the feasibility of these methods for future use in shielded cells
PPDC - Scientific Staff
Diversity and phylogenetic relationships within the spiral nematodes of Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae) as inferred from analysis of the D2-D3 expansion segments of 28S rRNA gene sequences. Identification and phylogenetic relationships within the stem nematode Ditylenchus dipsaci species complex (Tylenchida: Anguinidae) as inferred from analysis of the ITS-rDNA sequences
The role of the gut microbiota in nutrition and health
ties 73,74 and changes in gut motility 75,76 and energy Although most sugars are fermented by common or expenditure.77 Therefore, changes in the relative produc- converging pathways to yield SCFAs,96 some require tion rates of the major SCFAs by the colonic microbiota alternative routes. Studies that include patients at first presentationthe development of insulin resistance that is triggered or in remission can, therefore, provide valuable insights.by high-fat diets.127 Proinflammatory LPS is assumed to The gut microbiota is considered to have an impor-originate mainly from Gram-negative Proteobacteria in tant role in the prevention of sporadic colorectal cancerthe small intestine
Publications : 454 Life Sciences, a Roche Company
View Publication The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. View Publication Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp
simplex (without or with N) or Rlv alone (without N) and the two bacteria mixed together in a 1:1 ratio (without N); (b) Same treatment as above, but shoot length measurements were made 18 dpi; (c) Effect of Bacillus strains on pea root biomass. Taken together, these data demonstrate that an approach of mixing nitrogen-fixing rhizobia and bacteria with PGP and anti-fungal activity increases legume growth and biomass production
The essential membrane receptor ATPase SecA, and members of the integral membrane complex SecG, SecY, and SecD (responsible for release of the mature peptide) are present. Interestingly, guanine and adenine can also possibly serve as unique precursors of all purine nucleotides if the first 2 reactions of each pathway have reversibility
Revista Brasileira de Zootecnia - Recent developments in nucleic acid based techniques for use in rumen manipulation
New-generation DNA sequencing technologies (eg pyrosequencing) are now available which provide high throughput capacity and continuous sequence reads from 250-500bp in a cost-effective manner (Margulies et al., 2005). Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohydrates: effect of a yeast additive
Results A 16S rRNA gene clone library was constructed, and all sequences obtained from 161 clones showed affiliation with the phylum Firmicutes, with 160 sequences identified as Clostridiales and one sequence identified as unclassified Firmicutes. The samples processed in this study were not repeatedly thawed and analysed and we expect little loss of bacterial number and species compared to if the samples were mixed with glycerol before storing
The phenomenon may be widespread, since it was shown to occur among genotypically unrelated strains from different hospitals and different isolation dates. seven ESBL-producing clinical isolates collected during 2001 and three more recently collected isolates (Table 1), the API20E codes 5205773 (isolates GA1, GA2, GA3, MN2, MN3 and VGM), 5205753 (isolates DHJ1 and DHJ3) or 5204673 (isolates RA and DBH) were obtained
The Human Vaginal Bacterial Biota and Bacterial Vaginosis
As conditions in the vagina may be transient and dependent on numerous factors, most molecular studies offer a snapshot of the vaginal microbiota under specific conditions. Other bacteria detected in subjects with BV included Gardnerella vaginalis, Megasphaera, Leptotrichia, Dialister, Atopobium, and several bacterial vaginosis associated bacteria (BVABs) from the Clostridiales order
rRNA operon analysisIn addition to sequence analysis, DNA fingerprinting analysis based on 16S genes such as denaturing- or temperature gradient gel electrophoresis (DGGE, TGGE), single-stranded conformation polymorphism (SSCP), amplified ribosomal DNA restriction analysis (ARDRA) and terminal restriction fragment polymorphism analysis (T-RFLP) are widely used to differentiate microorganisms (Bouchet et al., 2008). In this manuscript, we discuss the limitations of 16S sequences in both applications and emphasize the need for alternative or complementary strategies.2
Evidence of Uncultivated Bacteria in the Adult Female Bladder
For pyrosequencing, barcoded degenerate primers targeting the hypervariable V1-V3 subregion of eubacterial 16S rRNA were employed, and the resulting amplicons were sequenced in multiplex by using the Roche 454 Titanium platform (6, 29, 31). On average, the UniFrac distance between the voided sample (V) and the vaginal swab (S) from the same participant was shorter than the distance between the voided and the TUC (C) samples from that same participant (participants 6 and 8)
Equal aliquots of the mock bacterial community were stored for 4 weeks at four different temperatures, extracted by two methods, and amplified in three PCR replicates. The mock bacterial community HM-280, though a mixture of bacterial cells with equal colony-forming units from each of the 22 species, was not quantified for true genome copy number for each component by the manufacturer
Conservative Fragments in Bacterial 16S rRNA Genes and Primer Design for 16S Ribosomal DNA Amplicons in Metagenomic Studies
The predicted primers reported in this study comprise nearly a full-set of primers for prokaryotic 16S rRNA genes and largely overlapped with known primers, regardless of any shift in positions. The accuracy of our prediction of the phylum specificity of the primers was affected by the numbers of orders and classes in a given phylum and the sequences available for these sub-phylum levels
Using a simple method, the primers have proven useful in identification of proteobacteria (Campylobacter, Enterobacter, Escherichia, Helicobacter, Klebsiella), grampositive bacteria (Mycobacterium, Staphylococcus, Streptococcus) and spirochetes (Borrelia) derived from clinical samples
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